Original Article
Safety and Immunogenicity of RTS,S/AS02D Malaria Vaccine in Infants
N Engl J Med 2008; 359:2533-2544December 11, 2008
- Abstract
Background
The RTS,S/AS malaria vaccine is being developed for delivery through the World Health Organization's Expanded Program on Immunization (EPI). We assessed the feasibility of integrating RTS,S/AS02D into a standard EPI schedule for infants.
Methods
In this phase 2B, single-center, double-blind, controlled trial involving 340 infants in Bagamoyo, Tanzania, we randomly assigned 340 infants to receive three doses of either the RTS,S/AS02D vaccine or the hepatitis B vaccine at 8, 12, and 16 weeks of age. All infants also received a vaccine containing diphtheria and tetanus toxoids, whole-cell pertussis vaccine, and conjugated Haemophilus influenzae type b vaccine (DTPw/Hib). The primary objectives were the occurrence of serious adverse events during a 9-month surveillance period and a demonstration of noninferiority of the responses to the EPI vaccines (DTPw/Hib and hepatitis B surface antigen) with coadministration of the RTS,S/AS02D vaccine, as compared with the hepatitis B vaccine. The detection of antibodies against Plasmodium falciparum circumsporozoite and efficacy against malaria infection were secondary objectives.
Results
At least one serious adverse event was reported in 31 of 170 infants who received the RTS,S/AS02D vaccine (18.2%; 95% confidence interval [CI], 12.7 to 24.9) and in 42 of 170 infants who received the hepatitis B vaccine (24.7%; 95% CI, 18.4 to 31.9). The results showed the noninferiority of the RTS,S/AS02D vaccine in terms of antibody responses to EPI antigens. One month after vaccination, 98.6% of infants receiving the RTS,S/AS02D vaccine had seropositive titers for anticircumsporozoite antibodies on enzyme-linked immunosorbent assay (ELISA). During the 6-month period after the third dose of vaccine, the efficacy of the RTS,S/AS02D vaccine against first infection with P. falciparum malaria was 65.2% (95% CI, 20.7 to 84.7; P=0.01).
Conclusions
The use of the RTS,S/AS02D vaccine in infants had a promising safety profile, did not interfere with the immunologic responses to coadministered EPI antigens, and reduced the incidence of malaria infection. (ClinicalTrials.gov number, NCT00289185.)
Media in This Article
Figure 1
Enrollment and Outcomes.Figure 2
Kaplan–Meier Curves Showing the Cumulative Incidence of at Least One Episode of Malaria Infection during the Study.Article Activity
- Article
Malaria persists as a major public health problem, and new tools for control of the disease are needed to facilitate the current renewed commitment for its control or elimination.1,2 The malaria vaccine contains the RTS,S antigen formulated with one of two adjuvant systems (AS), AS01 or AS02, and targets the pre-erythrocytic stage of Plasmodium falciparum parasite. This vaccine also has the potential to provide protection against infection with hepatitis B virus, since it contains the hepatitis B surface antigen. Studies conducted thus far show that the vaccine has a promising safety profile, is immunogenic, and confers partial protection against infection in adults who have not had malaria infection in the challenge model3,4 and in adults with partial immunity in the Gambia.5 A proof-of-concept study in children between the ages of 1 and 4 years in Mozambique also demonstrated protection against clinical malaria and severe disease lasting more than 18 months.6,7
The RTS,S/AS candidate malaria vaccine, which is being developed for infants and children in regions in sub-Saharan Africa in which malaria is endemic, will ideally be delivered through the Expanded Program on Immunization (EPI) of the World Health Organization. Therefore, the two main aims of the development plan are to demonstrate prevention of malaria, which occurs in infants who have reached about 4 months of age (when maternally acquired immunity wanes8) and to support the inclusion of the RTS,S/AS vaccine in the EPI, which has successfully expanded the coverage of basic vaccines across the developing world.9
The clinical development plan for the RTS,S/AS02D vaccine (with the letter D indicating the pediatric formulation) has followed a two-step approach after the proof-of-concept trial.6 As a first step, the vaccine was tested in infants in Mozambique with a staggered administration of malaria vaccine and EPI vaccines.10 This trial showed that the malaria vaccine candidate had a promising safety profile, was immunogenic, and conferred 65% protection against malaria infection in infants.10 We describe the results of the coadministration of this vaccine with EPI vaccines in infants living in an area of perennial malaria transmission in Tanzania.
Methods
Study Design
This phase 2B, single-center, double-blind, controlled trial was conducted between July 2006 and February 2008 by the Bagamoyo Research and Training Centre, a branch of the Ifakara Health Institute in Bagamoyo, Tanzania. The protocol was approved by the Ifakara Health Institute, the Western Institutional Review Board in the United States, the National Institute of Medical Research in Tanzania, the Institutional Review Board of the London School of Hygiene and Tropical Medicine, and the Swiss Tropical Institute through the local government ethics committee in Basel, Switzerland. The trial was undertaken in accordance with the provisions of the International Conference on Harmonisation and Good Clinical Practice guidelines and was monitored by the sponsor, GlaxoSmithKline Biologicals, which provided both the RTS,S/AS02D vaccine and the hepatitis B vaccine. Vaccine containing diphtheria and tetanus toxoids, whole-cell pertussis vaccine, and conjugated Haemophilus influenzae type b vaccine (DTPw/Hib) (TETRActHib) was purchased from Aventis Pasteur.
The design, conduct, and results of the trial were overseen by a formally constituted data and safety monitoring board, operating under a charter. Written informed consent in Swahili was obtained from parents of infants before study entry; parents who were not able to write indicated consent using a thumbprint, with a signature from a literate witness to the consent procedure. All authors vouch for the completeness and accuracy of the data presented. For further methodologic details, see the Supplementary Appendix, available with the full text of this article at www.nejm.org.
Study Vaccines
We randomly assigned infants to receive three doses of either the RTS,S/AS02D vaccine or the hepatitis B vaccine (Engerix-B) through intramuscular injection in the left anterolateral thigh and the DTPw/Hib vaccine through intramuscular injection in the right anterolateral thigh at 8, 12, and 16 weeks of age. Each dose of the RTS,S/AS02D vaccine (0.5 ml) contained 25 μg of RTS,S and the adjuvant system AS02D, as described previously.11 Oral polio vaccine was provided and administered at birth with sequential doses of DTPw/Hib.
Safety Assessments
After each vaccination, infants were observed for 1 hour for general adverse events. Trained field workers visited the children at home every day for the following 6 days to record solicited reports of adverse events. Unsolicited reports of adverse events were recorded for 30 days after each dose, and serious adverse events were recorded throughout the study with the use of the morbidity surveillance system in place at Bagamoyo District Hospital. In addition, all enrolled infants were visited monthly at home by field workers to maximize identification of serious adverse events. As part of safety monitoring at the initial screening, at 1 week after the first dose of a study vaccine, and at 1 month after the third dose, we measured hemoglobin, hematocrit, platelets, and white cells, along with creatinine for assessment of renal function and alanine aminotransferase and bilirubin for assessment of hepatic function. The intensity of symptoms was graded on a scale of 0 to 3, with higher scores indicating greater intensity. Grade 3 symptoms were defined as crying when the limb was moved or a spontaneously painful limb (local pain), injection-site redness or swelling measuring more than 20 mm in diameter, an axillary temperature of more than 39.0°C, or other symptoms preventing normal daily activities (for details, see the Supplementary Appendix).
Monitoring for Clinical Malaria Episodes
Surveillance for malaria infections by P. falciparum was undertaken by both active detection of infection and passive case detection. Any infant presenting with a documented fever (axillary temperature, ≥37.5°C) within the preceding 24 hours underwent a blood draw for the determination of malaria parasites. For active detection of infection, home visits were conducted every 2 weeks after the administration of the third dose of a study vaccine. Four weeks before the start of surveillance for malaria infection (i.e., 2 weeks before the third dose), asymptomatic parasitemia was cleared with artemether–lumefantrine, with the dose determined according to body weight. Each infant received a total of six doses of this drug during a 3-day period. The absence of parasitemia was confirmed by the analysis of a blood sample obtained 2 weeks later. Infants who continued to have parasitemia were retreated and excluded from the analysis. (For further details regarding the active detection of infection and determination of parasitemia, see the Supplementary Appendix.)
Laboratory Analysis
Antibody titers for anticircumsporozoite and anti–hepatitis B surface antigen were determined at screening and 1 month after the second and third doses of vaccine. Antibodies against diphtheria and tetanus toxins, polyribosylribitol phosphate for Hib, and Bordetella pertussis were measured at baseline and 1 month after the third dose of vaccine. The noninferiority of responses to hepatitis B, diphtheria, tetanus, Hib, and whole-cell pertussis were determined 1 month after the third dose (see the Supplementary Appendix for details).
Statistical Analysis
The analysis was based on a prospectively defined report and analysis plan. The primary end point for safety was the occurrence of serious adverse events during the first 9 months of the study in the intention-to-treat population, which included all infants for whom data were available. The primary end point of immunogenicity was the demonstration of noninferiority with respect to antibody responses to all antigens in the EPI vaccines at 1 month after the third dose of vaccine. This analysis was conducted in the per-protocol population for immunogenicity, which included all infants who could be evaluated — in other words, those who met all eligibility criteria, who had full compliance with the procedures (as defined in the protocol), who had no elimination criteria during the study, and for whom data concerning immunogenicity end-point measures were available. Noninferiority criteria were predefined and set to exclude more than a 10% decrease in protective antibody levels to diphtheria, tetanus, Hib, and hepatitis B surface antigen or to rule out a decrease by more than a factor of 1.5 in average antibody titers to whole-cell pertussis after vaccination. Secondary end points included further analyses of safety, reactogenicity, and immunogenicity and an estimation of vaccine efficacy against incident infections.
Vaccine efficacy was estimated for the per-protocol population (for details, see the Supplementary Appendix). Cases of infection were first or only infections with asexual P. falciparum, as detected by either active or passive means during the follow-up period, starting 14 days after the third dose of a study vaccine and continuing for approximately 7 months. The study evaluated efficacy against clinical malaria, which had a primary case definition of fever (axillary temperature, ≥37.5°C) with an asexual parasitemia of 500 parasites per microliter or more as an exploratory end point. This case definition had a reported sensitivity and specificity of more than 90%.12 A secondary case definition for clinical malaria included fever or a history of fever in the previous 24 hours plus any asexual P. falciparum parasitemia. The measure of person-years at risk was adjusted for absences from the study area and for the use of antimalarial drugs. Estimates of vaccine efficacy for the intention-to-treat population included all infants who had received at least one dose of a study vaccine. Measurement of the time at risk started at the administration of the first dose. Time at risk was not corrected for absences or for the use of antimalarial drugs.
Vaccine efficacy was defined as 1 minus the hazard ratio and was adjusted according to the village of residence and the distance to Bagamoyo District Hospital. The adjusted vaccine efficacy was assessed with the use of Cox regression models.
The sample size of 170 infants per study group was calculated to have a power of 90% to reach the noninferiority of the RTS,S/AS02D vaccine, as compared with the hepatitis B vaccine, with respect to EPI antigen responses after vaccination. The trial also had a power of 80% to detect a difference of 7 to 13% between the two study groups in reports of serious adverse events (for varying rates in the control subjects) and a power of more than 90% to detect a significant vaccine efficacy under the assumption of an attack rate of 50% in control subjects and of 45% true vaccine efficacy. A two-sided P value of less than 0.05 was considered to indicate statistical significance.
Results
Subjects
The first mother was screened on July 21, 2006. The first infant was enrolled on September 27, 2006, and the last infant was enrolled on May 4, 2007. A total of 378 infants were screened, and 340 were vaccinated with the first dose of a study vaccine (Figure 1Figure 1
Enrollment and Outcomes.). The same number of infants in each study group (153) completed the final study visit at 9 months.The demographic profiles of the group receiving the RTS,S/AS02D vaccine and the group receiving the hepatitis B vaccine in the intention-to-treat population were balanced in terms of sex, mean age, and distance from the health center (Table 1Table 1
Demographic Characteristics of the Subjects (Intention-to-Treat Population).). The mean age of infants when they received the first dose of a study vaccine was 7.8 weeks.Safety and Reactogenicity
From the time of the first vaccination until 9 months after the first dose, at least one serious adverse event was reported in 31 of 170 infants receiving the RTS,S/AS02D vaccine (18.2%; 95% confidence interval [CI], 12.7 to 24.9) and in 42 of 170 infants receiving the hepatitis B vaccine (24.7%; 95% CI, 18.4 to 31.9) (Table 2Table 2
Incidence of Serious Adverse Events, Unsolicited Reports of Adverse Events, and Solicited Reports of Injection-Site and General Adverse Events (Intention-to-Treat Population).). When serious adverse events caused by malaria were excluded, similar numbers of subjects in the two groups reported serious adverse events: 29 receiving the RTS,S/AS02D vaccine (17.1%) and 40 receiving the hepatitis B vaccine (23.5%).Pneumonia was the most frequently reported serious adverse event in both groups, occurring in 10 infants receiving the RTS,S/AS02D vaccine (5.9%; 95% CI, 2.9 to 10.6) and in 28 infants receiving the hepatitis B vaccine (16.5%; 95% CI, 11.2 to 22.9) (P=0.003). The next two most frequently reported serious adverse events were anemia (in two infants receiving the RTS,S/AS02D vaccine and in eight receiving the hepatitis B vaccine) and gastroenteritis (in eight infants receiving the RTS,S/AS02D vaccine and in five infants receiving the hepatitis B vaccine). All other serious adverse events occurred infrequently. One serious adverse event was fatal: a case of severe pneumonia and symptomatic seizures in an infant receiving the hepatitis B vaccine. Observers who were unaware of study-group assignments determined that none of the serious adverse events were related to vaccination.
Solicited reports of pain and swelling at the injection site occurred with a similar incidence in the two study groups (Table 2). No solicited reports of grade 3 injection-site symptoms were reported after the administration of either study vaccine. In comparison, the local reactogenicity associated with the DTPw/Hib vaccine was 85.0% of doses that were followed by pain and 3.8% of doses that were followed by swelling.
Among solicited reports, fever (axillary temperature, ≥37.5°C) and irritability were the most frequently reported general symptoms in the two vaccine groups (Table 2). Fever was reported more frequently after the administration of the RTS,S/AS02D vaccine with DTPw/Hib than after the administration of the hepatitis B vaccine with DTPw/Hib (29.6% and 13.6%, respectively). Most fevers were grade 1 or grade 2 in intensity (<39°C); only one grade 3 fever (≥39°C) was reported, after the third dose of hepatitis B vaccine.
Irritability, drowsiness, and loss of appetite were recorded after similar proportions of doses of the two vaccines; none of these symptoms were grade 3 in intensity.
During the first 30 days after vaccination, unsolicited reports of symptoms occurred in 80.6% of infants receiving the RTS,S/AS02D vaccine and in 82.9% of those receiving the hepatitis B vaccine (Table 2); the most common symptoms were cough (47.1% in both groups), rhinorrhea (32.9% and 42.9%, respectively), and pneumonia (28.8% and 31.8%, respectively). There was a trend toward a higher incidence of rash in recipients of the RTS,S/AS02D vaccine (7.1%; 95% CI, 3.7 to 12.0) than in recipients of the hepatitis B vaccine (0.6%; 95% CI, 0 to 3.2); none of these events were of severity grade 3. There was no other imbalance in other adverse events between the two study groups, and all events were those expected for the population. Two infants receiving the hepatitis B vaccine had an unsolicited report of an adverse event that was considered to be related to a study vaccine: injection-site erythema of moderate intensity and rash of mild intensity. Unsolicited reports of grade 3 events were infrequent and occurred in similar proportions in recipients of the RTS,S/AS02D vaccine and hepatitis B vaccine (4.1% and 9.4%, respectively). The most frequently reported grade 3 adverse event was pneumonia (2.4% and 6.5%, respectively).
Only 12 hematologic or biochemical values were outside the acceptable range in the two study groups. The abnormalities, which were all of grade 1 toxicity, included six tests of hemoglobin (in four recipients of the RTS,S/AS02D vaccine and in two recipients of the hepatitis B vaccine) and one test of alanine aminotransferase in a recipient of hepatitis B vaccine 1 week after the first dose and four tests of hemoglobin (one in a recipient of the RTS,S/AS02D vaccine and three in recipients of the hepatitis B vaccine) and one test of the white-cell count in a recipient of the RTS,S/AS02D vaccine 1 month after the third dose.
Immunogenicity Results
Noninferiority of the humoral responses to all EPI antigens was demonstrated in a comparison of the RTS,S/AS02D vaccine with the hepatitis B vaccine coadministered with DTPw/Hib, as indicated by the value of −10 or more for the lower limit of the 95% confidence interval of difference in seroprotection rates or a value of more than 0.66 for the ratio of geometric mean titers between the two groups. Rates of seroprotection and seropositivity were high for all antigens (>94%) (Table 3Table 3
Rates of Seropositivity or Seroprotection and Geometric Mean Titers for Key Antibodies at Baseline and after Dose 3 of a Study Vaccine Coadministered with DTPw/Hib Vaccines (Per-Protocol Population).).Before vaccination, the prevalence of maternally transferred antibodies against EPI antigens was relatively high, except for B. pertussis; however, geometric mean titers were low. After the full vaccination course, geometric mean titers for EPI antigens (apart from antibodies to hepatitis B surface antigen) tended to be lower among infants receiving the RTS,S/AS02D vaccine than among those receiving the hepatitis B vaccine. In the intention-to-treat population, 14 infants (4.1%) — 4 receiving the RTS,S/AS02D vaccine and 10 receiving the hepatitis B vaccine — did not reach seroprotective concentrations for all EPI antigens. All these children were revaccinated with the respective antigens.
At baseline, 33 of 141 children (23.4%) receiving the RTS,S/AS02D vaccine and 39 of 152 children (25.7%) receiving the hepatitis B vaccine were seropositive for anticircumsporozoite antibodies (≥0.5 enzyme-linked immunosorbent assay [ELISA] units per milliliter) with low titers (Table 3). One month after administration of the third dose of a study vaccine, 141 of 143 of infants (98.6%) receiving the RTS,S/AS02D vaccine were seropositive for anticircumsporozoite antibodies (geometric mean titer, 69.5; 95% CI, 53.9 to 89.6), as compared with 2 infants receiving the hepatitis B vaccine (1.4%). All infants receiving the RTS,S/AS02D vaccine were seroprotected against hepatitis B, as compared with 94.3% of those receiving the hepatitis B vaccine.
Efficacy
After the administration of the third dose of a study vaccine, 28 incident malaria infections involving the detection of any level of parasitemia were reported in the two study groups between day 14 and approximately 7 months (Table 4Table 4
Vaccine Efficacy during a Period from 14 Days to 7 Months after the Administration of Dose 3 of a Study Vaccine.). During this period, the incidence of the first malaria infection was 0.12 per person-year in infants receiving the RTS,S/AS02D vaccine and 0.29 per person-year in those receiving the hepatitis B vaccine. The crude vaccine efficacy against infection was 60.6% (95% CI, 10.4 to 82.6; P=0.03), which increased to 65.2% (95% CI, 20.7 to 84.7; P=0.01) after adjustment for the subject's area of residence and distance from the health center. Figure 2Figure 2Kaplan–Meier Curves Showing the Cumulative Incidence of at Least One Episode of Malaria Infection during the Study. shows Kaplan–Meier curves of the cumulative incidence of first malaria infections in the two study groups.The adjusted rates of vaccine efficacy were 58.6% (95% CI, −1.8 to 83.2) for the incidence of febrile malaria (the first or only episode of axillary fever of ≥37.5°C or a history of fever and any parasitemia) and 43.2% (95% CI, −47.1 to 78.0) for febrile malaria with a parasite-density threshold of more than 500 per microliter. Vaccine efficacy against febrile malaria (first or only episode of axillary fever of >37.5°C or a history of fever and any parasitemia) in the intention-to-treat population from first vaccination was 41.8% (95% CI, −32.9 to 74.6; P=0.20). Exploration of the relationship between antibody titers and the risk of infection showed that a doubling of anticircumsporozoite titers corresponded to a 16% reduction in the risk of infection and that there was a significant difference in titers between infants receiving the RTS,S/AS02D vaccine who were not infected and those who were infected (74.8 vs. 17.8 ELISA units per milliliter, P=0.03).
Discussion
In our trial, the use of the RTS,S malaria vaccine, formulated in the AS02 adjuvant system, when coadministered to infants with other routinely delivered EPI immunizations, did not pose any obvious safety concerns and did not interfere with the immunogenicity of the multiple coadministered antigens. The results are in keeping with those of a trial in which the administration of the RTS,S/AS02D vaccine to Mozambican infants was staggered with EPI vaccinations.10 Low-grade fever was reported more frequently in infants receiving the RTS,S/AS02D vaccine (29.6%) than in the control group receiving the hepatitis B vaccine (13.6%). There were no cases of high-grade fever in infants receiving the RTS,S/AS02D vaccine, and there was no significant difference in the number of other solicited and unsolicited reports of adverse events between the two study groups. Since hospitalizations for pneumonia were more common in the control group, the malaria vaccine candidate may reduce important indirect consequences of malaria.
At the time of the first vaccination, about 25% of the infants were seropositive with low concentrations of anticircumsporozoite antibodies, which were probably acquired transplacentally. After 3 months, the anticircumsporozoite antibodies had almost disappeared in the control group but were higher in almost all RTS,S/AS02D vaccine recipients. The concentrations of the anticircumsporozoite titer in our study were lower than those observed in the staggered administration and among children between the ages of 1 and 4 years,6,10 which suggests that the EPI vaccines may have interfered with the anticircumsporozoite responses but had not prevented the conferring of protection. These data suggest an association between the level of anticircumsporozoite antibody titer and the risk of P. falciparum infection, in keeping with previous studies that have found a relationship between the level of anticircumsporozoite antibodies and efficacy against infection but not against clinical episodes.6,10 The response induced to hepatitis B surface antigen by the RTS,S/AS02D vaccine was higher than that of the licensed vaccine.
We also evaluated the feasibility of incorporating the RTS,S/AS02D vaccine into the standard EPI vaccination schedule in terms of immunogenicity. Our study followed a previous trial10 in which either the RTS,S/AS02D vaccine or the hepatitis B vaccine was given, staggered with the same DTPw/Hib vaccine that we used in our trial. Responses to antigens in the EPI vaccines were similar in the two comparator groups in which DTPw/Hib vaccine was administered either alone or in combination with hepatitis B vaccine. The geometric mean titers and seroprotection rates were broadly similar between a group receiving simultaneous administration of the EPI vaccines and a group receiving delayed (by 2 weeks) administration, with seroprotection rates of 100% in the latter group for diphtheria, tetanus, pertussis, and Hib and of 98.5% for hepatitis B surface antigen.
Criteria of noninferiority for all EPI antigens were predefined in the protocol. Although responses that were induced to most components of the DTPw/Hib vaccine were slightly lower for infants receiving the RTS,S/AS02D vaccine than for those receiving the hepatitis B vaccine, seroprotective levels for both groups and all antigens were high, and all noninferiority criteria were met.
In an earlier trial of the RTS,S vaccine in children,6 efficacy against breakthrough infections was associated with reductions in both mild and severe malaria episodes. The rate of protection of 65% against new infections that we observed is encouraging and is similar to the observations in the earlier trial with staggered administration in Mozambican infants.10 However, rates of infection were substantially lower in our trial, and the finding of the previous trial with respect to efficacy against febrile episodes was not confirmed.
The low rate of detection of infection through active surveillance is likely to be a result of improved malaria control associated with distribution of bed nets, along with the close follow-up and improved clinical care of the infants our in study. Given that the infants in this trial had the best access to existing preventive tools and treatment of malaria in Tanzania, these results indicate the added benefit of a malaria vaccine within an integrated approach for the control and elimination of malaria.
Further development of the RTS,S/AS vaccine in a large phase 3 trial is warranted. If licensed and recommended for inclusion in the EPI schedule, the RTS,S/AS vaccine could become an effective component of an integrated strategy to control malaria.
Supported by the Program for Appropriate Technology in Health (PATH) Malaria Vaccine Initiative and by GlaxoSmithKline Biologicals.
Ms. Carter and Dr. Villafana report being employees of the Malaria Vaccine Initiative, which supports the development and testing of several malaria vaccines; Drs. Demoitié, Dubois, Leach, Lievens, Vekemans, Cohen, and Ballou, being employees of GlaxoSmithKline Biologicals at the time the study was performed; Drs. Dubois, Leach, Cohen, and Ballou, having an equity interest in GlaxoSmithKline; Drs. Cohen and Ballou, being listed as inventors of patented malaria vaccines, although neither reports holding a patent for a malaria vaccine.
No other potential conflict of interest relevant to this article was reported.
This article (10.1056/NEJMoa0807773) was published at www.nejm.org on December 8, 2008.
We thank all the children and parents who participated in this study; the Bagamoyo communities and their leaders; the entire staff at Bagamoyo Research and Training Centre of Ifakara Health Institute, Bagamoyo District Hospital, and the dispensaries in the study area; Zaria Said and Richard Kamata, study counselors at the Swiss Tropical Institute, Basel, for providing key support to this trial and acting as site partners; Christoph Hatz for serving as a liaison between the trial team and the Swiss institutional review boards; Christine Walliser and Christian Wirz for their administrative and logistic support; Hans-Peter Marti for providing assistance with laboratory procedures; Rahiya Shariff for her coordination of the communications team; our colleagues in other trial sites supported by the Malaria Vaccine Initiative through the Bill and Melinda Gates Foundation, particularly the team from Centro de Investigaçao em Saude de Manhiça at Manhiça, Mozambique, which provided support and advice for the implementation of the trial; the staff of the Malaria Project Team at GlaxoSmithKline Biologicals, particularly Nathalie Annez, Delphine Beauport, Sarah Benns, Philippe Dehottay, Issam Jaimai, Isabelle Ramboer, Shantala Rao, Christine Swysen, Joelle Thonnard, Marie Chantal Uwamwezi, Wendy Valinski, and Laurence Vigneron; Christian Loucq, Melinda Moree, and Regina Rabinovich for their hard work and dedication to the project; Karim Manji for providing continuous support and guidance; Malcolm Molyneux, chair of the data and safety monitoring board, for his instruction and support; Andrew Kitua, director of the National Institute of Health at the Ministry of Health and Social Welfare; and the staff of the Contract Laboratory Services of South Africa for providing invaluable support.
Source Information
From the Bagamoyo Research and Training Centre of Ifakara Health Institute, Bagamoyo, Tanzania (S.A., R.O., O.J., F.M., C.M., S.O., A.U., H.M., A.J., N.S., M.S., T.A., D.M.S., M.T.); Swiss Tropical Institute, Basel, Switzerland (R.O., T.A., M.T.); Muhimbili University of Health and Allied Sciences, Dar es Salaam, Tanzania (S.K.); London School of Hygiene and Tropical Medicine, London (D.M.S.); Program for Appropriate Technology in Health (PATH) Malaria Vaccine Initiative, Bethesda, MD (T.C., T.V.); GlaxoSmithKline Biologicals, Rixensart, Belgium (M.-A.D., M.-C.D., A.L., M.L., J.V., J.C.); and Bill and Melinda Gates Foundation, Seattle (W.R.B.).
Address reprint requests to Dr. Abdulla at the Bagamoyo Research and Training Centre, Ifakara Health Institute, Box 74, Bagamoyo Tanzania, or at sabdulla@ihi.or.tz.
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- Citing Articles (59)
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D. Lys Guilbride, Patrick D.L. Guilbride, Pawel Gawlinski. (2012) Malaria's deadly secret: a skin stage. Trends in Parasitology
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Obinna N. Nnedu, Michael P. O'Leary, Daniel Mutua, Beth Mutai, Mina Kalantari-Dehaghi, Al Jasinskas, Rie Nakajima-Sasaki, Grace John-Stewart, Phelgona Otieno, Xiaowu Liang, John Waitumbi, Francis Kimani, David Camerini, Philip L. Felgner, Judd L. Walson, Adam Vigil. (2011) Humoral immune responses to Plasmodium falciparum among HIV-1-infected Kenyan adults. PROTEOMICS - Clinical Applications 5:11-12, 613-623
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