Correspondence
Herpesvirus-Like DNA Sequence in Angiosarcoma in a Patient without HIV Infection
N Engl J Med 1996; 334:540-541February 22, 1996
- Article
To the Editor:
Recent studies have demonstrated the presence of a new gamma herpesvirus–like DNA sequence, designated Kaposi's sarcoma–associated herpesvirus (KSHV) or human herpesvirus-8, in different forms of Kaposi's sarcoma in patients infected with the human immunodeficiency virus (HIV)1 and in uninfected patients.2 KSHV sequences were also detected in proliferative lesions other than Kaposi's sarcoma (AIDS-related body-cavity–based lymphomas,3 Castleman's disease,4 and various proliferative skin lesions5) in immunocompromised patients. Nador et al. (Oct. 5 issue)6 reported finding KSHV DNA in a body-cavity–based lymphoma in an HIV-negative patient. We report detecting the KSHV sequence in another non–Kaposi's sarcoma tumor from a nonimmunocompromised patient.
A 63-year-old woman presented with a one-year history of a small, reddish-blue discoloration of the skin on her right cheek, resembling a hematoma, that had developed into a rapidly growing angiomatous tumor two weeks before admission. The patient was HIV-negative, had not received any immunosuppressive therapy, and had no clinical signs of immunodeficiency. After radical excision of the tumor, the clinical diagnosis of angiosarcoma was confirmed by histologic examination.
DNA was extracted from the paraffin-embedded tissue, and the sample was tested for the presence of KSHV sequences by the polymerase chain reaction (PCR) with primers specific for the 233-base-pair (bp) KS330233 fragment, as described previously.1,2 The results are shown in Figure 1Figure 1
PCR Amplification of KSHV DNA with KS330233 Primers.. The 233-bp amplification product was identified in the angiosarcoma sample, as well as in a sample of Kaposi's sarcoma from an HIV-negative patient. The specificity of the band was confirmed by hybridization to a previously sequenced KSHV probe from a sample of Kaposi's sarcoma. Cloning and direct sequencing of the PCR product revealed that the KSHV sequence found in the angiosarcoma sample was nearly identical to the originally described sequence in Kaposi's sarcoma. It differs only in two C→T substitutions at positions 1033 and 1168.Our findings represent at least the second report of a non–Kaposi's sarcoma tumor that was KSHV-positive in an HIV-negative, nonimmunocompromised patient. Our result suggests that this novel viral agent may be implicated in the pathogenesis of other malignant angiomatous tumors as well. This would weaken the hypothesis that KSHV is only lymphotropic and would support the assumption that the virus also has angiotropic properties. Further studies are necessary, however, to confirm the suspected causative role of KSHV in the development of these different lymphomatous and angiomatous neoplasms.
6 ReferencesRolland Gyulai, M.D.
Lajos Kemény, M.D., Ph.D.
Mária Kiss, Ph.D.
Albert Szent-Györgyi Medical UniversityÉva Ádám, Ph.D.
Ferenc Nagy, Ph.D.
Hungarian Academy of SciencesAttila Dobozy, M.D., Ph.D., D.Sc.
Albert Szent-Györgyi Medical University, H-6701 Szeged, Hungary1
Chang Y ,Cesarman E ,Pessin MS , et al. Identification of herpesvirus-like DNA sequences in AIDS-associated Kaposi's sarcoma. Science 1994;266:1865-1869
CrossRef | Web of Science | Medline2
Kemény L, Gyulai R, Kiss M, et al. Herpesvirus-like nucleic acid sequences in patients with Eastern European sporadic Kaposi's sarcoma. J Invest Dermatol (in press).
3
Cesarman E ,Chang Y ,Moore PS ,Said JW ,Knowles DM . Kaposi's sarcoma-associated herpesvirus-like DNA sequences in AIDS-related body-cavity-based lymphomas. N Engl J Med 1995;332:1186-1191
Full Text | Web of Science | Medline4
Dupin N ,Gorin I ,Deleuze J ,Agut H ,Huraux J-M ,Escande J-P . Herpes-like DNA sequences, AIDS-related tumors, and Castleman's disease. N Engl J Med 1995;333:798-798
Web of Science | Medline5
Rady PL ,Yen A ,Rollefson JL , et al. Herpesvirus-like DNA sequences in non-Kaposi's sarcoma skin lesions of transplant patients. Lancet 1995;345:1339-1340
CrossRef | Web of Science | Medline6
Nador RG ,Cesarman E ,Knowles DM ,Said JW . Herpes-like DNA sequences in a body-cavity-based lymphoma in an HIV-negative patient. N Engl J Med 1995;333:943-943
Full Text | Web of Science | Medline
- Citing Articles (28)
Citing Articles
1
C Pestoni, C Paredes-Suarez, C Peteiro, J Toribio. (2005) Early detection of cutaneous angiosarcoma of the face and scalp and treatment with placitaxel. Journal of the European Academy of Dermatology and Venereology 19:3, 357-359
CrossRef2
Lauren Hammock, Amy Reisenauer, Wayne Wang, Cynthia Cohen, George Birdsong, Andrew L Folpe. (2005) Latency-associated nuclear antigen expression and human herpesvirus-8 polymerase chain reaction in the evaluation of Kaposi sarcoma and other vascular tumors in HIV-positive patients. Modern Pathology 18:4, 463-468
CrossRef3
James A. Ramirez, William B. Laskin, Joan Guitart. (2005) Lymphangioma-like Kaposi sarcoma. Journal of Cutaneous Pathology 32:4, 286-292
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Holger Schmid, Christian Zietz. (2005) Human herpesvirus 8 and angiosarcoma: analysis of 40 cases and review of the literature. Pathology 37:4, 284-287
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I. Schneider. (2002) To the Hundredth Anniversary of the Death of Moriz Kaposi (1837-1902). Acta Microbiologica et Immunologica Hungarica 49:4, 501-506
CrossRef6
Marco Gessi, Paola Cattani, Nicola Maggiano, Giovanni Scambia, Libero Lauriola, Franco O. Ranelletti, Riccardo Ricci. (2002) Demonstration of Human Herpesvirus 8 in a Case of Primary Vaginal Epithelioid Angiosarcoma by In Situ Hybridization, Electron Microscopy, and Polymerase Chain Reaction. Diagnostic Molecular Pathology 11:3, 146-151
CrossRef7
Ralf Gutzmer, Markus Kaspari, Rudolf A. Herbst, Alexander Kapp, Peter Kiehl. (2002) Absence of HHV-8 DNA in hobnail hemangiomas. Journal of Cutaneous Pathology 29:3, 154-158
CrossRef8
Anne E. Allan, Toru Shoji, Ning Li, Ann Burlage, Bret Davis, Jag Bhawan. (2001) Two Cases of Kaposi's Sarcoma Mimicking Stewart-Treves Syndrome Found to be Human Herpesvirus-8 Positive. The American Journal of Dermatopathology 23:5, 431-436
CrossRef9
Kyoung-Ae Jang, Se-Jin Ahn, Jee-Ho Choi, Kyung-Jeh Sung, Kee-Chan Moon, Jai-Kyoung Koh, Yong-Hee Shim. (2001) Polymerase chain reaction (PCR) for human herpesvirus 8 and heteroduplex PCR for clonality assessment in angiolymphoid hyperplasia with eosinophilia and Kimura's disease. Journal of Cutaneous Pathology 28:7, 363-367
CrossRef10
PALACIOS, UMBERT, CELADA. (1999) Correspondence. British Journal of Dermatology 140:1, 170-171
CrossRef11
Pfrommer, Tebbe, Tidona, WOlfer, Krengel, Zeichardt, Zouboulis, Orfanos. (1998) Progressive HHV-8-positive classic Kaposi's sarcoma: rapid response to interferon alpha-2a but persistence of HHV-8 DNA sequences in lesional skin. British Journal of Dermatology 139:3, 516-519
CrossRef12
Ning Li, William K. Anderson, Jag Bhawan. (1998) Further confirmation of the association of human herpesvirus 8 with Kaposi's sarcoma. Journal of Cutaneous Pathology 25:8, 413-419
CrossRef13
T COOK, S FOSKO. (1998) Unusual cutaneous malignancies. Seminars in Cutaneous Medicine and Surgery 17:2, 114-132
CrossRef14
Joachim Krischer, Olivier Rutschmann, Bernard Hirschel, Sonia Vollenweider-Roten, Jean-Hilaire Saurat, Marc Pechère. (1998) Regression of Kaposi’s sarcoma during therapy with HIV-1 protease inhibitors: A prospective pilot study. Journal of the American Academy of Dermatology 38:4, 594-598
CrossRef15
Attila Juhász, Eva Remenyik, Krisztina Szarka, György Veress, János Hunyadi, Lajos Gergely. (1998) Consistent polymerse chain reaction–single-strand conformation polymorphism pattern of human herpesvirus-8 in the course of classical Kaposi's sarcoma assumes its clonal origin. Journal of Medical Virology 54:4, 300-304
CrossRef16
Martinez-Escribano, del Pino Gil-Mateo, Miquel, Ledesma, Aliaga. (1998) Human herpesvirus 8 is not detectable by polymerase chain reaction in angiosarcoma. British Journal of Dermatology 138:3, 546-547
CrossRef17
S.R Porter, L Di Alberti, N Kumar. (1998) Human herpes virus 8 (Kaposi’s sarcoma herpesvirus). Oral Oncology 34:1, 5-14
CrossRef18
R. Gyulai, L. Kemeny, M. Kiss, S. Nagy, É. Áám, F. Nagy, A. Dobozy. (1997) Human herpesvirus 8 DNA sequences in angiosarcoma of the face. British Journal of Dermatology 137:3, 467-468
CrossRef19
M DICTOR. (1997) Human herpesvirus 8 and Kaposi's sarcoma1. Seminars in Cutaneous Medicine and Surgery 16:3, 181-187
CrossRef20
M. Takata, N. Hatta, K. Takehara, H. Fujiwara. (1997) Absence of human herpesvirus-8 DNA in angiosarcmas and other skin tumours in immnocompetent patients, and in graft-versus-host disease in the immunosuppressed recipients of bone marrow transplants. British Journal of Dermatology 137:1, 156-157
CrossRef21
Daniel A. Arber, John G. Strickler, Yuan-Yuan Chen, Lawrence M. Weiss. (1997) Splenic Vascular Tumors: A Histologic, Immunophenotypic, and Virologic Study. The American Journal of Surgical Pathology 21:7, 827-835
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E. BERTI, A.V MARZANO, I. DECLEVA, G. BESTETTI, M. PIZZUTO, L. POIREL, M. CAPRA, C. PARRAVICINI, M. CORBELLINO. (1997) Simultaneous onset of primary cutaneous B-cell lymphoma and human herpesvirus 8-associated Kaposi's sarcoma. British Journal of Dermatology 136:6, 924-929
CrossRef23
M.C. Steiner, M.E.F. Smith, M.F. Spittle. (1997) A patient with classical Kaposi's sarcoma and angiosarcoma: Bad luck or a common aetiology?. Clinical Oncology 9:3, 186-188
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Omeed M. Memar, Peter L. Rady, Randall M. Goldblum, Stephen K. Tyring. (1997) Human Herpesvirus-8 DNA Sequences in a Patient with Pemphigus Vulgaris, but Without HIV Infection or Kaposi's Sarcoma.. Journal of Investigative Dermatology 108:1, 118-119
CrossRef25
Joseph G Sinkovics. (1996) Contradictory concepts in the etiology and regression of kaposi’s sarcoma. Pathology & Oncology Research 2:4, 249-267
CrossRef26
R. Inagi, H. Kosuge, S. Nishimoto, K. Yoshikawa, K. Yamanishi. (1996) Kaposi's sarcoma-associated herpesvirus (KSHV) sequences in premalignant and malignant skin tumors. Archives of Virology 141:11, 2217-2223
CrossRef27
Robert J. Biggar, Charles S. Rabkin. (1996) THE EPIDEMIOLOGY OF AIDS-RELATED NEOPLASMS. Hematology/Oncology Clinics of North America 10:5, 997-1010
CrossRef28
Rolland Gyulai, Lajos Kemény, Éva Ádám, Ferenc Nagy, Attila Dobozy. (1996) HHV8 DNA in angiolymphoid hyperplasia of the skin. The Lancet 347:9018, 1837
CrossRef
